Tools

Three interactive tools I built, all running entirely in your browser — nothing is uploaded, nothing is installed, and no sequence ever leaves your machine. Start with the DNA Sequence Lab for quick analysis, step through a CRISPR‑Cas9 edit, then take the sequence into the Protein Lab. Free to use, including in teaching.

DNA Sequence Lab

Paste a DNA sequence to analyse it, scan for SpCas9 targets, check for Golden Gate Type IIS sites, or run a local alignment.

CRISPR‑Cas9 editing, step by step

Pick a target gene and an edit type, then run it. The guide RNA anneals to the protospacer, Cas cuts 3 bp upstream of the NGG PAM, and the sequence is repaired with a lesion — an indel (knockout) or a substitution — with the bases colour-coded throughout.

Protein Lab

Translate DNA and find ORFs, compute physicochemical properties (MW, pI, charge, GRAVY, extinction coefficient), plot Kyte–Doolittle hydropathy, and scan for common sequence motifs — all in the browser.