Research Officer · MARDI, Malaysia
I edit crop genomes, and take apart the enzymes that feed them.
I am a plant biotechnology researcher at the Biotechnology & Nanotechnology Research Centre, MARDI. My work runs at two scales: rewriting a handful of bases in a rice or tomato genome with CRISPR‑Cas9, and understanding how a single microbial enzyme — a beta‑propeller phytase — is built and behaves.
Between the two sits the computational work that makes them talk to each other: guide design, phylogenetics, structural modelling, and the biosafety documentation that lets an edited plant leave the growth room.
What I work on
CRISPR in rice & tomato
Dual guide RNA constructs in Golden Gate vectors for transgene‑clean edits — blast resistance in rice, fruit quality in tomato.
02Phytase biotechnology
A beta‑propeller phytase from a local Bacillus siamensis isolate, from molecular screening through expression and assay.
03Computational genomics
Marker‑gene phylogenies, homology models, and variant mapping that link sequence to what we measure at the bench.
Tools I built
Three genome‑engineering tools that run entirely in your browser — no install, no upload, nothing leaves your machine. I wrote them to teach the methods behind my own work, and they are free for anyone to use.
- DNA Sequence Lab — composition and Tm, SpCas9 target scanning, Type IIS site checks, local alignment.
- CRISPR‑Cas9 walkthrough — pick a gene and an edit type, then watch the cut and repair happen base by base.
- Protein Lab — translation and ORFs, MW/pI/GRAVY, hydropathy plots, motif scanning.