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Research blog
Field notes from the bench and the terminal
Longer-form writing on genome editing, molecular biology and the small methodological decisions that quietly decide whether an experiment works.
Reading Cas12a crRNA self-processing from a plasmid map
Cas12a processes its own guide array through direct repeats, which means a single-transcript multiplex build needs neither Csy4 nor tRNA spacers. Here is how to spot the direct-repeat sequence and overhangs in a vector map.
Cleaner Ni-NTA: fixing broad SDS-PAGE bands
Two quiet culprits behind smeared phytase bands — running the gel too slowly and washing with too little imidazole. Raising the wash to 40–50 mM and the voltage to the recommended setting sharpened everything.
Choosing a substitution model with ModelFinder
Before you trust a species assignment, let BIC pick the model and let UFBoot2 tell you how much to believe the branches. A short, practical walk-through.
Notes on regenerating edited rice
Regeneration, not delivery, is usually the bottleneck. A few things that improved recovery from edited callus in our hands.
What I look for in a genome-editing talk
A personal checklist — controls, off-target strategy, and whether the outcome analysis actually supports the headline.
A minimal, honest phytase activity assay
Why we keep phosphate out of the buffers, hold calcium constant, and read at 360 nm — and the standard curve you cannot skip.
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